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Centromere and kinetochore proteins in yeasts and human

The following tables list the proteins involved in centromeric chromatin and kinetochore assembly in budding yeast, fissing yeast, and human. Proteins that appear on the same line are deemed to be orthologs.

This page is intended to serve as a quick reference only. For more information, refer to the appropriate litterature and data banks (each protein name below is a link to the corresponding entry in UniProt).

Centromeric Proteins

The Constitutive Centromere-Associated Network (CCAN) is a complex of proteins permanently associated with centromeric chromatin. It is also known as the “Ctf19p complex” in S. cerevisiae and the “Sim4 complex” in S. pombe. This table and the next one are mostly taken from Przewloka & Glover, 2009.

CCAN Proteins
Budding yeast Fission yeast Human
CENP-O / COMA subcomplex
Ctf19p Fta2 CENP-P
Okp1p
Mcm21p Mal2 CENP-O
Ame1p
Fta7 CENP-Q
CENP-R
CENP-H subcomplex
Mcm16p Fta3 CENP-H
Ctf3p Mis6 CENP-I
Sim4 CENP-K
CENP-T/W subcomplex
CENP-T
CENP-W
Other proteins
Iml3p (aka Mcm19p) Mis17 CENP-M
Chl4p Mis15 CENP-N
Mcm22p
Nkp1p
Nkp2p
Fta1 CENP-L
Fta4 CENP-U
Fta5
Fta6
CENP-S

Beside the CCAN, other proteins are associated with the centromeric chromatin (notably the centromeric histone H3 variant, listed first in the table below).

Other Centromeric Proteins
Budding yeast Fission yeast Human
Cse4p Cnp1 CENP-A1
Abp1, Chb1, Cbh2 CENP-B
Mif2p Cnp3 CENP-C
Cbf1p

Kinetochore Proteins

The KMN (Knl1–Mis12–Ndc80) network is believed to be the core module of the kinetochore. It is made of three complexes conserved from yeasts to primates (Welburn & Cheeseman, 2008; Santaguida & Musacchio, 2009).

KMN Network
Budding yeast Fission yeast Human
Knl1 (aka Spc105 or Spc7) complex
Spc105p Spc7 KNL1 (aka CASC5)
Kre28p Zwint-1
Mis12 (aka MIND) complex
Mtw1p Mis12 MIS12
Nnf1p Nnf1 PMF1
Nsl1p Mis14 NSL1
Dsn1p Mis13 DSN1
Ndc80 complex
Ndc80p Ndc80 NDC80 (aka Hec1)
Nuf2p Nuf2 Nuf2
Spc24p Spc24 Spc24
Spc25p Spc25 Spc25

CenH3 Loading Pathways

The following proteins are not necessarily associated with centromeres and kinetochores but are involved in the loading of centromeric histone CenH3/CENP-A into the centromeric chromatin (Mellone et al., 2009).

CenH3 Loading Pathways
Budding yeast Fission yeast Human
Scm32 Sim13 HJURP
Sim3
Mis16 RbAp46, RbAp48
Mis18 Mis18α, Mis18β
MIS18BP1

Spindle Checkpoint Proteins

The Chromosome Passengers Complex (CPC) is a protein complex transiently localized in centromeres in metaphase, where it is involved in the correction of mis-attached kinetochores (Ruchaud et al., 2007).

CPC Proteins
Budding yeast Fission yeast Human
Ipl1p Ark1 Aurora B
Sli15p Pic1 INCENP
Bir1p Bir1 Survivin
Nbl1p Mug118 Borealin
  1. Aka CenH3 in all species (Centromeric H3)
  2. Mizuguchi et al., 2007
  3. Pidoux et al., 2009